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2 edition of Analysis of anaerobic benzene biodegradation. found in the catalog.

Analysis of anaerobic benzene biodegradation.

Sandra L. Little

Analysis of anaerobic benzene biodegradation.

by Sandra L. Little

  • 94 Want to read
  • 3 Currently reading

Published .
Written in English


About the Edition

Benzene is a known carcinogen and benzene contamination of groundwater presents a threat to those reliant on aquifers for drinking water. Aerobic benzene biodegradation is well understood yet the pathway for anaerobic benzene degradation is unknown. This study examined the enrichment of anaerobic benzene-degrading microbial communities and reports a maximum benzene degradation rate of 104 muM/d and a maximum specific rate of benzene degradation 5.2 muM/mg protein/day. Methylation of benzene to toluene was previously observed yet in this study the methanogenic culture observed did not produce toluene during benzene degradation, and degraded existing toluene. Diethyl ether extraction coupled to GC/MS analysis using a DB WAX column was not effective in determining if 13C carbonate was the source of the methyl group for benzene methylation to toluene. This study reinforces that a great deal remains unknown about these enrichment cultures and changes in degradation rates do not always follow expected patterns.

The Physical Object
Pagination117 leaves.
Number of Pages117
ID Numbers
Open LibraryOL19216326M
ISBN 100494071370

benzene is the most robust in almost all field studies, as a carcinogen and therefore the most toxic et al., ; Farhadian M., ) It is within this context that our work studied the biodegradation of benzene by pure locally isolated strains already having the . Biodegradation is defined as the biologically catalyzed reduction in complexity of chemical compounds [ 1 ]. Indeed, biodegradation is the process by which organic substances are broken down into smaller compounds by living microbial organisms [ 2 ]. When biodegradation is complete, the process is called "mineralization". However, in most cases Cited by:

Microbial community analysis of benzene-degrading sediments and enrichment cultures using molecular techniques has sug-gested that a functional role in anaerobic benzene degradation is played by members of the family Geobacteraceae under Fe(III)-reducing conditions (33) and by members of the familyCited by: for benzene and for toluene. Introduction Biological treatment of contaminated aquifers is receiving increasing interest. Where applicable, in situ biodegradation of the toxic petroleum hydro- carbons benzene and toluene can serve as a cost effective groundwater cleanup method (Barker et.

Anaerobic biodegradation of substances to metabolic acids may significantly lower the pH (Parsons )—for example, the production of H + ions during reductive dechlorination or the production of volatile fatty acids from electron donor fermentation tends to decrease the pH of groundwater (ITRC ). A pH below 5 or 4 standard units could.   However, anaerobic benzene biodegradation has been documented under various conditions. Although benzene biomineralization has been demonstrated with nitrate1, Fe(III)2,3,4,5, sulphate6,7 or CO28 Cited by:


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Analysis of anaerobic benzene biodegradation by Sandra L. Little Download PDF EPUB FB2

The benzene ring moiety is found in biological compounds accounting for ~25% of the land-based organic matter on Earth. Hence, the biosynthesis and biodegradation of aromatic ring compounds constitutes an important part of natural carbon cycle. Microorganisms capable of using aromatic compounds as carbon and energy sources can be found in a range of very Author: Weimin Sun, Valdis Krumins, Donna E.

Fennell, Lee J. Kerkhof, Max M. Häggblom. Biodegradation Results by Treatment Analysis of + SIP Anaerobic Benzene Biodegradation.

Anaerobic Benzene Degradation •Reported under all terminal electron accepting processes •Degraders likely not ubiquitous •May be slow with long lag times and/or inhibited by co-contaminants. Dou J, Ding A, Liu X, Du Y, Deng D, Wang J () Anaerobic benzene biodegradation by a pure bacterial culture of Bacillus cereus under nitrate reducing conditions.

J Environ Sci 22(5)– CrossRef Google ScholarAuthor: Bhawana Pathak, Shalini Gupta, M. Fulekar. Anaerobic benzene biodegradation by a pure bacterial culture of Bacillus cereus under nitrate reducing conditions. Journal of Environmental Sciences22 (5), Cited by:   Signature Metabolite Analysis to Determine In Situ Anaerobic Hydrocarbon Biodegradation.

, DOI: /_ Carsten Jobelius, Fritz H. Frimmel, Christian Zwiener. Mass spectrometric screening and identification of acidic metabolites in fulvic acid fractions of contaminated by: Monoaromatic hydrocarbons such as benzene, toluene, ethylbenzene, and xylene (BTEX) are widespread contaminants in groundwater.

We examined the anaerobic degradation of BTEX compounds with amorphous ferric oxide as electron by:   In some highly enriched strictly anaerobic cultures, benzene has been described to be mineralized cooperatively by two or more different organisms.

Despite great efforts, the biochemical mechanism by which the aromatic ring of benzene is activated in the absence of oxygen is still not fully elucidated; methylation, Cited by: Brazilian Journal of Microbiology () ISSN BENZENE, TOLUENE AND XYLENE BIODEGRADATION BY PSEUDOMONAS PUTIDA CCMI Marcelo Henrique Otenio 1; Maria Teresa Lopes da Silva 2; Maria Luiza Oliveira Marques 2; José Carlos Roseiro 2; Ederio Dino Bidoia 1* 1Instituto de Biociências, Departamento de Bioquímica e Microbiologia.

Abstract. To date, evidence for the degradation of benzene under anaerobic conditions has been established only in few studies under field and laboratory conditions.

Recently, we demonstrated the mineralization of benzene under sulfate-reducing conditions in a large-scale column experiment at a field site by balancing electrons (Vogt et by: Description, Properties, and Degradation of Selected Volatile Organic Compounds Detected in Ground Water— A Review of Selected Literature By Stephen J.

Lawrence Abstract This report provides abridged information describing the most salient properties and biodegradation of.

Biodegradation of Benzene Under Anaerobic Condition. Global industrialization has largely expanded the edges of petroleum hydrocarbon (PHC) exploration. A large amount of various hydrocarbons are introduced into the environment during the stages of oil extraction, refinement, storage, transportation and disposal.

Conclusions and Recommendations Anaerobic Biodegradation of BTEX 1. Benzene, toluene, ethylbenzene and the xylene isomers are anaerobically biodegradable under ambient subsurface conditions using ferric iron, sulfate and/or carbon dioxide as terminal electron acceptors. A distinct order of biodegradation is often observed, with toluene being.

Anaerobic benzene and naphthalene degradation is performed by microbes belonging to different phyla. Metabolite analysis of cultures suggest as initial activation reaction either methylation, hydroxylation, or carboxylation for benzene, and methylation or carboxylation for naphthalene.

Proteogenomic studies indicate carboxylation of benzene and naphthalene. Non-substitute aromatic hydrocarbons Cited by: anaerobic biodegradation of compounds in groundwater [Toxic Substances Control Act (TSCA) final test rule of J ; 53 Fed.

Reg.40 CFR section“Anaerobic microbiological transformation rate data for chemicals in the subsurface environment”]. Anaerobic biodegradation of aromatic compounds P lothimani, G Kalaichelvan, The central metabolite of anaerobic degradation is invariably co-A thio-esters of benzoic acid or hydroxy benzoic acid.

The benzene ring undergoes various substilUtion and addition reactions to form chloro', nitro', methyl' compounds. Cited by: biodegradation e.g., electron donors, electron acceptors, nutrients, etc. • Bioaugmentation: addition of beneficial microorganisms to improve biodegradation Injection of KB-1 ® at a site in Florida each liter of KB-1 has Billion Dhc cellsFile Size: 1MB.

Nie et al. () reported 50% efficiency for LAS removal by adsorption, rather than biodegradation, using a submerged anaerobic membrane bioreactor technique. The low efficiency of the process was due to the effects on the methanogenic : Mayara Franco Costa, Ana Maria de Oliveira, Enio Nazaré de Oliveira Junior.

This study reports on benzene and toluene biodegradation under different dissolved oxygen conditions, and the goal of this study is to evaluate and model their removal. Benzene and toluene were tested for obligate anaerobic degradation in batch reactors with sulfate as the electron acceptor.

A group of sulfate-reducing bacteria capable ofFile Size: 5MB. Dechloromonas strain RCB has been shown to be capable of anaerobic degradation of benzene coupled to nitrate reduction.

As a continuation of these studies, the metabolic versatility and hydrocarbon biodegradative capability of this organism were investigated. The results of these revealed that in addition to nitrate, strain RCB could alternatively degrade benzene Cited by:   The total amount of 13 CO 2 produced from 13 C 6 benzene degradation was determined by measuring the 13 CO 2 / 12 CO 2 isotope ratio in the headspace.

The headspace sample (1 mL) was diluted in a mL serum vial filled with helium and stored until analysis. The diluted gas samples ( mL) were injected to a GC/C/IRMS system consisting of a TRACE Cited by:.

Anaerobic benzene biodegradation is an alternative that can have economic and sustainability benefits (Coates, Chakraborty, & McInerney, ).

Although benzene biodegradation has been.EPA uses anaerobic biodegradation rates in the subsurface fate and transport model. The Agency considers that although anaerobic biodegradation is not the slowest activity, it is less likely that data collected under anaerobic conditions would lead to an overestimation of the degradation rate.

In general, the concentrationsFile Size: KB. The goal of our study was to establish anaerobic benzene-degrading enrichment cultures starting from the colonized sand particles and lava granules inside the in situ microcosms after exposure.

This was done in order to test the hypothesis that anaerobic benzene-degrading microorganisms living at the Zeitz site prefer a sessile life by: